Michaelis–Menten Enzyme Kinetics Calculator

Reaction velocity from Vmax, Km and substrate concentration; catalytic efficiency.

Results

Velocity v—
Fraction of Vmax—
kcat = Vmax/[E]—
kcat/Km—

How to use the Michaelis–Menten Enzyme Kinetics Calculator

  1. Enter or select: Vmax, Km, Substrate [S].
  2. Fill in what you know of: Enzyme concentration [E] — leave the unknown empty.
  3. The results (Velocity v, Fraction of Vmax, kcat = Vmax/[E], kcat/Km) are calculated instantly as you type.
  4. Use “Copy link” to share the calculation with the same input values.

Formula

  • v = Vmax·[S] / (Km + [S])